Isolation and staging of horse seminiferous tubules by transillumination
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چکیده
منابع مشابه
Isolation of Germ Cell Golgi Apparatus from Seminiferous Tubules of Rat Testes
Intact Golgi apparatus have been isolated with good purity from rat testis by a simplified sucrose gradient technique. The procedure is inherently selective in that most of the Golgi apparatus in the isolate are from germ cells in late spermatocyte or early spermatid development. No Sertoli cell Golgi apparatus are present in the fraction. Biochemical analyses showed that the enzyme N-acetylglu...
متن کاملO-6: Maturation of Spermatogenic Cells in Artificial Seminiferous Tubules
Background This study aimed to investigate whether Artificial Seminiferous Tubules (AST) coated with testicular extra cellular matrix (ECM) support maturation of spermatogenic cells from immature testes. MaterialsAndMethods Medical grade tubes coated with testicular ECM were used. Testes from immature mice were loaded in the concentration of 10000- 20000/ml. Tubes were then immersed in germ cel...
متن کاملHistomorphometric evaluation of treatment of rat azoosper-mic seminiferous tubules by allotransplantation of bone marrow-derived mesenchymal stem cells
Objective(s): Bone marrow-derived mesenchymal stem cells (BM-MSCs) potentials make them appropriate for cell therapy including ability of differentiation and release of anti-inflammatory cytokines and growth factors secreta. For treatment of azoospermia to induce proliferation and differentiation of germ cells, MSCs transplantation has been introduced. The aim of the present experimental case-c...
متن کاملThe Morphology of the Seminiferous Tubules of Mammalia
At the Cleveland meeting of the American Association of Anatomists, (1912-1913), Curtis1 reported on wax plate reconstructions of the seminiferous tubules of the white mouse, presenting figures of a model of an entire tubule having an actual length of somewhat over 13 em. The tubule completely reconstructed presents the form of an arch, the two ends of the arch lying in close proximity, each te...
متن کاملEgress of sperm autoantigen from seminiferous tubules maintains systemic tolerance.
Autoimmune responses to meiotic germ cell antigens (MGCA) that are expressed on sperm and testis occur in human infertility and after vasectomy. Many MGCA are also expressed as cancer/testis antigens (CTA) in human cancers, but the tolerance status of MGCA has not been investigated. MGCA are considered to be uniformly immunogenic and nontolerogenic, and the prevailing view posits that MGCA are ...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1990
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0890689